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KMID : 0380620090410030313
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Korean Journal of Food Science and Technology
2009 Volume.41 No. 3 p.313 ~ p.319
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Enhancement of Immune Activity of Spirulina maxima byLow Temperature Ultrasonification Extraction
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Oh Sung-Ho
Han Jae-Gun
Ha Ji-Hye
Kim Young
Jeong Myeong-Hoon
Kim Seung-Seop
Jeong Hyang-Suk
Choi Geun-Pyo
Park Uk-Yeon
Kang Do-Hyung
Lee Hyeon-Yong
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Abstract
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The marine microalga Spirulina maxima was extracted using water or ethanol at 100 or 80oC and byultrasonification in water at 60oC. The ultrasonification technique generated the highest yield (19.8%). To be therapeuticallyuseful, the extraction should yield a product with low cytotoxicity and high immunity against skin infections. Thecytotoxicity of all extracts (1.0mg/mL) was below 25%. Moreover, the cytotoxicity of the extract generated byultrasonification was 5%. Extracts prepared in the described manners could inhibit hyaluronidase activity by up to 40%compared to the control. Increased growth of human B, T and NK cells and an increase in cytokine secretion wereobserved, confirming the interrelationship between both human immune and skin immune activity. The extract prepared byultrasonification increased the growth of human B, T and NK cells up to 10.3×104 cells/mL, 11.3×104 cells/mL and19.1×104 cells/mL, respectively. The extract prepared by ultrasonification also greatly increased the secretion of both IL-6and TNF-α. Moreover, it was estimated that protein, Na and leucine occupy a high ratio. Accordingly, this study hasconfirmed that extracts prepared as described have the potential to effectively increase skin immunity.
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KEYWORD
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Spirulina maxima, cytotoxicity, cytokine, NK cell, hyaluronidase
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